Update on our task at hand:

Yay, so much for Nicholas doing the updating on the blog.

Well anyway, at the task at hand, we're currently researching on natural herbs that are able to neturalize/kill the bacterias.

The bacterias are 

E-Coli was found on the notes/Currency.

Lactobacillus acidophilus was found on the toilet seat.

taphylococcus aureus was found on the sink.

Anyway, we've decided on using a couple of different herbs to start off first.

We've decided to use the

Clitoria Leaves,

Aloe Vera Leaves,

Mint Leaves and

Pandang Leaves

if i remember correctly.

So we've already taken the first step by using Clitoria leaves and Clitoria Flowers.

We've done this by firstly grinding each of the samples, than adding distilled water in so we can get the extracts and sort of "juice" of the plant. 

The Clitoria leaves ended up with a sort of green water, which was than filtered.

The Clitoria flowers ended up with a bluish water, (Which has quite a nice colour to it), and it was than filtered.

We also nurtured more colonies of bacteria from notes, (Therefore mainly e-coli?)

which is currently in the incubator. (We haven't started on nurturing bacteria from the toilet yet)

The notes used were old $2 notes and plastic ones.

We used the same technique to make the colonies of bacteria, only this time of plate streaking we first took a cotton bud and covered the bud (All 360 degrees) with the bacteria. Than we streaked the agar plate once, followed by disposing the cotton bud. Then we took a plastic inoculation loop (We didn't have a metal one). We used the plastic inoculation loop and streaked once at the end of the first streak we made, spreading the colony of bacteria. We than disposed the plastic loop (To keep it sterile. If we used a metal one, we'd have to heat the loop) We than repeated the step of the plastic loop twice-thrice more.

That's about all there is.

General Report

I have finally done up the general report, which summarizes EVERYTHING!

Although with this general report, do note that everything inside is NOT finalized yet! Please do take time and read through everything carefully as i've spent 3 hours on it.

Here's the link to download it:

http://www.box.net/shared/2zqrzvp5kl

Bacteria

I forgot to add on the results.

E-Coli was found on the notes/Currency.

Lactobacillus acidophilus was found on the toilet seat.

taphylococcus aureus was found on the sink.

I hope i'm right. Can't really remember.

Research on bacteria

This is the research on the bacteria that Ms Menon and Justin wanted.

Staphylococcus aureus is a spherical bacterium, frequently found in the nose and the skin of the person. About 20% of the population are long-term carriers of S.aureus. S.aureus can cause a range of illnesses from minor skin infections, such as pimples.

Escherichia Coli is a gram negative bacterium that is commonly found in the lower intestine of warm-blooded animals. Most E.Coli strains are harmless, but some can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls.

Lactobacillus acidophilus is one species in the genus Lactobacillus. Some research has indicated that L. acidophilus may provide additional health benefits, including improves gastrointestinal function, a boosted immune system, and a decrease in the frequency of vaginal yeast infections. Some people report L. acidophilus provides relief for indigestion and diarrhea.

Notes for the sciene fair

UPDATED:

Like I promised, after our CA, I would upload the screenies. Here it is.

Well, we went to the biotech fair a few weeks ago.
Due to the upcoming CA (Tomorrow!), we've not been very active on the blog. This will probably be the last update until the end of CA, so you can be sure that we'll be updating it frequently after the CA. Anyway, this are the biotech notes that we've taken down.


Thanks Nicholas for the notes, which he complied into a word document.

Here's the file link:
http://www.box.net/shared/2kvjjdvsn6


Like i said, after the CA, you can expect more things to be updated. So right now this file is only available to download. I'll post it up onto the blog using screen capture soon enough.

The procedures for Grain Staining

Apparatus used :

• Metal Inoculation Loop
• Glass Slide
• 4 Grain Stains; Iodine, Safranin, De-colorizer and Methyl Violet
• Bunsen Burner
• Distilled Water

Procedures :

1. Heat the Metal Inoculation Loop over a Bunsen Burner
   
2. Collect some bacteria and place it on the glass slide
3. Wash the Metal Inoculation Loop and heat it again
4. Use the Metal Inoculation Loop to collect a drop of Distilled Water
5. Position the drop of Water unto the middle of the Glass Slide
6. Evaporate the water over the Bunsen Burner
7. Stain the bacteria by using Methyl Violet and Iodine for 1 min, Followed by using the De-colorizer and Safranin for 10 seconds

Our progress on bacteria.

So well, we've already started our investigations and such. We've already taken the school toilet and currencies and tested them out in the science lab. As we speak, we're growing the bacteria on the alga- thingy. The one with alot of nutrients. (Haha, i don't know the name of it. :) ) So i'll update the results again on next coming monday where we'll get the results.