The procedures for Grain Staining

Apparatus used :

• Metal Inoculation Loop
• Glass Slide
• 4 Grain Stains; Iodine, Safranin, De-colorizer and Methyl Violet
• Bunsen Burner
• Distilled Water

Procedures :

1. Heat the Metal Inoculation Loop over a Bunsen Burner
   
2. Collect some bacteria and place it on the glass slide
3. Wash the Metal Inoculation Loop and heat it again
4. Use the Metal Inoculation Loop to collect a drop of Distilled Water
5. Position the drop of Water unto the middle of the Glass Slide
6. Evaporate the water over the Bunsen Burner
7. Stain the bacteria by using Methyl Violet and Iodine for 1 min, Followed by using the De-colorizer and Safranin for 10 seconds